Hansen, K., Hindersson, P., Strandberg Pedersen, N.: Measurement of antibodies to the Borrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. Journal of Clinical Microbiology 1985, 21: 819–825. T., Granström, M., Hederstedt, B., Sköldenberg, B.: Diagnosis of spirochetal meningitis by enzyme-linked immunosorbent assay and indirect immunofluorescence assay in serum and cerebrospinal fluid. P., Plikaytis, B.: Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J.: Isolation of Borrelia spirochetes from patients in Texas. E.: The spirochetal etiology of Lyme disease. A.: Erythema chronicum migrans and Lyme arthritis: the enlarging clinical spectrum. P.: Cardiac involvement in Lyme disease: manifestations and management. Hovmark, A., Åsbrink, E., Olsson, I.: The spirochetal etiology of lymphadenosis benigna cutis solitaria. Åsbrink, E., Hovmark, A., Hederstedt, B.: The spirochetal etiology of acrodermatitis chronica atrophicans Herxheimer. Yale Journal of Biology and Medicine 1984, 57: 491–497. Stiernstedt, G., Sköldenberg, B., Vandvik, B., Hederstedt, B., Gårde, A., Kolmodin, G., Jörbeck, H., Svenungsson, B.: Chronic meningitis and Lyme disease in Sweden. Deutsche Medizinische Wochenschrift 1984, 109: 92–97. C., Hartung, S., Runne, U.: Spirochäten-Ätiologie der Erythema-chronicum-migrans Krankheit. P.: Lymc disease - a tick-borne spirochetosis? Science 1982, 216: 1317–1319.Īckermann, R., Kabatzki, J., Boisten, H. In conclusion, Western blot does not seem to be the method of choice for screening purposes in a routine laboratory but can be used as a complement to ELISA for serodiagnosis in patients with disease of short duration.īurgdorfer, W., Barbour, A. However, the specificity for current disease was not improved by Western blot. Both patients and controls lived in an area endemic for Lyme borreliosis and some ELISA negative but Western blot positive controls were thought to have been previously exposed to Borrelia burgdorferi. Western blot was more sensitive than ELISA, the difference being most pronounced in sera from patients with neurological disease for four weeks or less. Eight of 44 (18 %) controls with meningitis/encephalitis of non-borrelia etiology had positive IgM and/or IgG immunoblots and 4 of 44 (9 %) had positive IgM and/or IgG ELISA titers in serum. Fifty-three of 68 (78 %) patients with neuroborreliosis had positive IgM and/or IgG immunoblots and 40 of 68 (59 %) had positive IgM and/or IgG ELISA titers in serum. The usefulness of Western blot in the serological diagnosis of Lyme borreliosis was evaluated compared with an ELISA using a whole cell sonicate antigen.
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